Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

Figure 4

Extracellular vesicles derived from mesenchymal stem/stromal cells induce keratinocyte and fibroblast migration. (a) Phase contrast images of human keratinocytes cultured in DMEM in the absence (KSFM) or presence of extracellular vesicles (KSFM + EVs) derived from mesenchymal stem/stromal cells (MSCs) in 0, 12, and 24 h after scratch. Scale bar = 200 μm. (b) Relative areas affected by the scratch wound at the three time points of the experiments. Significantly smaller scratch wound area was observed in 24 h after the scratch following the exposure to MSC EVs, indicating faster keratinocyte migration. Data are presented as the mean ± standard deviation of three independent experiments. Statistical significance of differences is indicated as follows: . (c) Phase contrast images of human fibroblasts cultured in DMEM in the absence (DMEM) or presence of extracellular vesicles (DMEM + EVs) derived from mesenchymal stem/stromal cells (MSCs) in 0, 12, and 24 h after scratch. Scale bar = 200 μm. (d) Relative areas affected by the scratch wound at the three time points of the experiments. Significantly smaller scratch wound area was observed in 24 h after the scratch following the exposure to MSC EVs, indicating faster fibroblast migration. Data are presented as the mean ± standard deviation of three biological independent experiments.

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