Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

Figure 7

Effects of extracellular vesicles derived from mesenchymal stem/stromal cells on AKT phosphorylation are independent of miR-205. (a) Graphs illustrate relative levels of miR-205 in human fibroblasts and keratinocytes cultured in standard culture medium or in the medium containing EVs derived from mesenchymal stem/stromal cells (MSCs). Relative expression of miR-205 was higher in cells exposed to MSC EVs. qPCR data were normalized using the 2^−ΔΔCt method. Data are presented as the mean ± standard deviation of three independent experiments. Statistical significance of differences is indicated as follows: . (b) Quantification of miR-205 relative expression levels by qPCR in fibroblasts (left graph) and keratinocytes (right graph) from the following experimental groups: nontreated (NT), exposed only to lipofectamine only (lipofectamine), transfected with scramble miR (scramble), transfected with synthetic miR-205 (miR-205 mimic), transfected with siRNA for miR-205 (siRNA), and, finally, transiently silenced for miR-205 and exposed to extracellular vesicles from mesenchymal stem/stromal cells (siRNA + EVs). Data are presented as the mean ± standard deviation. Statistical significance of differences is indicated as follows: . (c) Representative Western blot gels illustrating bands for total and phosphorylated AKT (p-AKT) from experimental groups described previously. (d) Densitometry analysis of the p-AKT/total AKT ratio in lysates of cells from different experimental groups. Data are presented as the mean ± standard deviation of three biological independent experiments. Statistical significance of differences is indicated as follows: ; .

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