Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

Figure 8

Effects of extracellular vesicles on the migration of fibroblasts and keratinocytes do not require miR-205. (a) Phase contrast images of scratch wound straight after the scratch was made (zero) and in 24 h after that in fibroblasts (left graph) and keratinocytes (right graph) from the following experimental groups: nontreated (NT), exposed only to lipofectamine only (lipofectamine), transfected with scramble miR (scramble), transfected with synthetic miR-205 (miR-205 mimic), transfected with siRNA for miR-205 (siRNA), and, finally, transiently silenced for miR-205 and exposed to extracellular vesicles from mesenchymal stem/stromal cells (siRNA + EVs). Scale bar = 200 μm. (b) Relative areas affected by the scratch wound in 24 h in all experimental groups. Data are presented as the mean ± standard deviation of three biological independent experiments. Statistical significance of differences is indicated as follows: ; .

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