Neuropilin 1 Mediates Keratinocyte Growth Factor Signaling in Adipose-Derived Stem Cells: Potential Involvement in Adipogenesis

Figure 5

Evaluation of differentiation pathway activation by KGF. (a) Sustained phosphorylation of ERK was assessed by Western blot analysis with a phospho-specific ERK monoclonal antibody (pERK) on ASC whole cell lysates, treated or not with 20 ng/ml KGF for 24 h. Levels of total ERK were assessed by blotting with an ERK2-specific antibody and served as loading control. (b) Phosphorylation of Akt was assessed by Western blot analysis with a phospho-specific Akt monoclonal antibody (pAkt) on ASC whole cell lysates, treated or not with 20 ng/ml KGF for 5 and 30 min. Levels of total Akt were assessed by blotting with an Akt-specific antibody and served as loading control. (c) Phosphorylation of Rb was assessed by Western blot analysis with a phospho-specific Rb monoclonal antibody (pRb) on ASC whole cell lysates, treated or not with 20 ng/ml KGF for 5 and 30 min. Levels of total Rb were assessed by blotting with a Rb-specific antibody and served as loading control. The images are representative of at least three independent experiments.

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