Amniotic Epithelial Cells Accelerate Diabetic Wound Healing by Modulating Inflammation and Promoting Neovascularization

Figure 6

hAEC-CM promoted phenotype switch of macrophages toward M2-like in vitro. (a) Changes of macrophages in morphology when treated with IFN-γ and TNF-α with or without hAEC-CM. Complete medium without IFN-γ and TNF-α was treated as the control medium. (b) Protein expression levels of iNOS and CD206 when treated with the control medium, IFN-γ + TNF-α, or IFN-γ + TNF-α + hAEC-CM were measured by Western blot. Data was shown as means ± SD; ; ; ; and ns: no significance; scale bars: 50 μm (a).

(a)

(b)