IFNα-Expressing Amniotic Fluid-Derived Mesenchymal Stem Cells Migrate to and Suppress HeLa Cell-Derived Tumors in a Mouse Model

Figure 2

Tropism of administered AF-MSCs for tumors in a mouse model. (a) Immunohistochemistry (IHC) showing CD90 expression in AF-MSCs. The tumors were collected and examined at day 1, day 3, and day 7 after the last injection. Untreated tumor stained for CD90 is used as a negative control. Bars, 25 or 50 μm. (b) IHC of AF-MSCs in the liver and spleen. Very few MSCs were observed. Bar, 25 μm. (c, d) Observation of CM-Dil-labeled AF-MSCs and IFNα-AF-MSCs (in red) in the eGFP-HeLa (in green) tumors by fluorescence microscopy. Bars, 10, 25, or 50 μm. HeLa cells that expressed eGFP (eGFP-HeLa) were injected subcutaneously into the backs of nude mice () at 1 × 10⁷/ml/mouse. AF-MSCs and IFNα-AF-MSCs were labeled with the red fluorescent dye CM-Dil, and 10 days following the initial inoculation of HeLa cells, 5 × 10⁶/ml of either MSCs, or IFNα-AF-MSCs were injected into the tail vein of the tumor-bearing mice every 5 days. IHC and fluorescence microscopy were used to observe the infiltration of AF-MSCs.

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