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Stem Cells International
Volume 2018, Article ID 1340341, 9 pages
Research Article

Inflammatory Stimuli Significantly Change the miRNA Profile of Human Adipose-Derived Stem Cells

1Department of Urology, Peking University First Hospital, Beijing 100034, China
2Institute of Urology, Peking University, Beijing 100034, China
3Department of Ophthalmology, Peking University First Hospital, Beijing 100034, China
4National Urological Cancer Center, Beijing 100034, China
5Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing 100034, China

Correspondence should be addressed to Jian Lin; moc.361@jbnaijnil and Liqun Zhou; moc.anis@liamnuqiluohz

Received 10 July 2017; Revised 8 October 2017; Accepted 22 October 2017; Published 28 January 2018

Academic Editor: Renke Li

Copyright © 2018 Zicheng Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Human adipose-derived stem cells (hASCs) have been shown to have immunoregulatory properties in many studies. However, the mechanisms remain unknown. miRNAs are associated with many cellular processes, including immune responses. Thus, we hypothesized that miRNAs act as immunoregulators when hASCs are stimulated by inflammatory environments. Methods. A set of cytokines was used to stimulate the hASCs in the cytokine group, while no cytokines were used to stimulate the cells in the normal group. A microarray was used to obtain the miRNA expression profile of hASCs, and RT-PCR was used to validate the miRNAs that were differentially expressed between the two groups. Target genes were predicted using online databases, and KEGG analysis was performed to identify the pathways enriched by the target genes of all the differentially expressed miRNAs. Results. Five miRNAs were significantly upregulated, and 2 miRNAs were downregulated in the cytokine group compared with the normal group. We identified several immune-related pathways that are targeted individually or collectively by those miRNAs. Conclusion. Inflammatory stimuli changed the miRNA expression profile of hASCs. miRNAs may play a pivotal role in the immune response in hASCs and may be targets through which the immunoregulatory functions of hASCs can be enhanced.