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Stem Cells International
Volume 2018, Article ID 1393607, 18 pages
https://doi.org/10.1155/2018/1393607
Research Article

Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy

11st Department of Propaedeutic Surgery, University of Athens, School of Medicine, “Hippocration” Hospital, V. Sofias Avenue 114, 11527 Athens, Greece
2Laboratory of Cell Proliferation & Ageing, National Center for Scientific Research “Demokritos”, Neapoleos 27 Street, 15341 Athens, Greece
31st Department of Pathology, School of Medicine, University of Athens, Tetrapoleos 18, 11527 Athens, Greece
4Experimental Research Center, ELPEN, Marathonos Avenue 95, 19009 Athens, Greece

Correspondence should be addressed to Konstantinos G. Apostolou; moc.liamg@uolotsopa.sonitnatsnok

Received 10 September 2017; Accepted 6 December 2017; Published 14 March 2018

Academic Editor: Shimon Slavin

Copyright © 2018 Konstantinos G. Apostolou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Adipose tissue stem cells (ADSCs) present a promising therapeutic method to alleviate liver failure (LF). The purpose of this prospective study was to evaluate the efficacy of undifferentiated ADSC transplantation on liver regeneration and on the expression of liver regeneration- and liver-specific genes, following 60% partial hepatectomy (PHx). Methods. Sixty female rats were subjected to PHx and were transplanted with 106 or 2 × 106 ADSCs, either into the portal vein (PV) or into the hepatic parenchyma. Animals of the control group were not transplanted and served as controls. Animals were sacrificed on the 4th, the 7th, or the 15th postoperative day (POD). Results. The transplanted ADSCs were successfully engrafted into the liver parenchyma and ameliorated the histopathologic damage on the 7th and 15th POD. All transplanted animals demonstrated a significantly higher liver regeneration rate on the 4th and 7th POD, compared with the control group. The expression of hepatocyte growth factor, α-fetoprotein, tyrosine aminotransferase, hepatocyte nuclear factor 4a, and cytochrome P450 1A2 was significantly upregulated, compared with the control group. Conclusions. Although undifferentiated, ADSC transplantation significantly enhanced the liver regeneration process. These findings may be proven clinically valuable, especially in cases of acute LF.