Stem Cells International

Research Article

Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy

Table 3

Liver histological analysis scoring.


Animal	Subgroup ID¹	Congestion	Vacuolization	Necrosis	Inflammation	Total score²

CN1-1	CN1	1	0	0	0	1
CN1-2	CN1	1	0	1	1	3
CN1-3	CN1	1	1	1	1	4
CN1-4	CN1	1	0	0	0	1
CN1-5	CN1	1	1	1	3	6
CN2-6	CN2	1	1	0	0	2
CN2-7	CN2	1	1	1	0	3
CN2-8	CN2	1	1	0	1	3
CN2-9	CN2	0	0	0	0	0
CN2-10	CN2	2	2	3	3	10
CN3-11	CN3	1	1	1	1	4
CN3-12	CN3	1	0	1	0	2
CN3-13	CN3	0	1	0	0	1
CN3-14	CN3	0	0	1	0	1
CN3-15	CN3	1	0	1	0	2
S1-1	S1	0	0	0	0	0
S1-2	S1	0	0	0	0	0
S1-3	S1	0	0	0	0	0
S1-4	S1	0	0	0	0	0
S1-5	S1	0	0	0	0	0
S2-6	S2	0	0	0	0	0
S2-7	S2	0	0	0	0	0
S2-8	S2	0	0	0	0	0
S2-9	S2	0	0	0	0	0
S2-10	S2	0	0	0	0	0
S3-11	S3	0	0	0	0	0
S3-12	S3	0	0	0	0	0
S3-13	S3	0	0	0	0	0
S3-14	S3	0	0	0	0	0
S3-15	S3	0	0	0	0	0
Α1-1	A1	1	0	2	2	5
Α1-2	A1	0	3	1	1	5
Α1-3	A1	0	3	1	1	5
Α1-4	A1	0	1	0	0	1
Α1-5	A1	1	3	1	1	6
Α2-6	A2	0	1	0	0	1
Α2-7	A2	0	0	0	0	0
Α2-8	A2	0	0	0	0	0
Α2-9	A2	1	0	1	2	4
Α2-10	A2	0	0	0	0	0
Α3-11	A3	0	0	0	0	0
Α3-12	A3	0	0	0	0	0
Α3-13	A3	0	0	0	0	0
Α3-14	A3	0	0	0	0	0
Α3-15	A3	0	0	0	0	0
B1-1	B1	0	0	0	0	0
B1-2	B1	0	1	0	0	1
B1-3	B1	0	1	1	1	3
B1-4	B1	0	0	0	0	0
B1-5	B1	0	1	0	0	1
B2-6	B2	0	0	0	0	0
B2-7	B2	0	0	0	0	0
B2-8	B2	0	0	0	0	0
B2-9	B2	0	0	0	0	0
B2-10	B2	0	0	0	0	0
B3-11	B3	0	0	0	0	0
B3-12	B3	0	0	0	0	0
B3-13	B3	0	0	0	0	0
B3-14	B3	0	0	0	0	0
B3-15	B3	0	0	0	0	0
C1-1	C1	0	0	0	0	0
C1-2	C1	0	1	0	0	1
C1-3	C1	1	0	1	1	3
C1-4	C1	1	2	2	1	6
C1-5	C1	0	2	2	1	5
C2-6	C2	0	0	0	0	0
C2-7	C2	0	0	0	0	0
C2-8	C2	0	0	0	0	0
C2-9	C2	0	0	0	0	0
C2-10	C2	0	0	0	0	0
C3-11	C3	1	0	0	1	2
C3-12	C3	0	1	0	0	1
C3-13	C3	0	0	0	0	0
C3-14	C3	1	0	0	1	2
C3-15	C3	0	0	0	0	0
D1-1	D1	0	0	0	0	0
D1-2	D1	0	1	0	0	1
D1-3	D1	1	1	1	1	4
D1-4	D1	1	0	1	1	3
D1-5	D1	0	0	0	0	0
D2-6	D2	0	1	0	0	1
D2-7	D2	0	0	1	0	1
D2-8	D2	0	1	0	0	1
D2-9	D2	0	0	0	0	0
D2-10	D2	0	0	0	0	0
D3-11	D3	0	0	0	0	0
D3-12	D3	0	1	0	0	1
D3-13	D3	0	0	0	0	0
D3-14	D3	0	0	0	0	0
D3-15	D3	0	0	0	0	0

Liver histological analysis for the following parameters: sinusoidal congestion, vacuolization of hepatocyte cytoplasm, parenchymal necrosis, and inflammation, with the total score representing the sum of all parameters for each individual animal. Each parameter was graded numerically as follows: congestion, vacuolization, and inflammation: 0 = none, 1 = minimal, 2 = mild, 3 = moderate, and 4 = severe. The numerical graduation for necrosis was as follows: 0 = nonnecrotic cells, 1 = single-cell necrosis, 2 ≤ 30% necrosis, 3 ≤ 60% necrosis, and 4 ≥ 60% necrosis. ¹The subgroup ID is depicted in Table 1 (experimental design). ²Total score represents the sum of all parameters for each individual animal.