Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

Figure 1

hADMSC immunophenotype and characterization of adipocyte differentiation. Positive and negative surface markers of MSCs and adipocyte differentiation marker hFATP-1 in (a). Cell cycle and G1 arrest of adipocytes in (b). hADMSC representative morphology at 4x magnification in (c) and 40x magnification in (d). Adipocyte differentiation after 14 days evidenced by Oil Red O staining. (e) and (g) are representative images at 20x and 40x magnification, respectively. Light microscopy images at day 14 of differentiation are represented in (f) and (h), corresponding to 20x and 40x magnification, respectively. The increase in the relative lipid accumulation between hADMSCs and adipocytes was determined by Student’s t-test, (i).

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