Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
DNA repair capacity of H2O2 treatment in human adipose-derived mesenchymal stem cells (hADMSCs). DNA damage induced and remnant after 24 h posttreatment (PT), expressed as Olive tail moment (OTM) corresponding to DNA breaks and alkali labile sites in (a) (mean ± SD). DNA-Fpg sites (7,8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil) in (b) (mean ± SD). Percentage of DNA repair capacity of DNA breaks and alkali labile sites in (c) (percentage ± SD) and percentage of DNA-Fpg sites’ repair capacity in (d) (percentage ± SD). 0 PT = control PT; 50 PT = 50 μM H2O2 PT; 100 PT = 100 μM H2O2 PT; 150 PT = 150 μM H2O2 PT; and 200 PT = 200 μM H2O2 PT. All data were obtained in 3 independent duplicate experiments (). Kruskal-Wallis was performed with one-way ANOVA on ranks, and all pairwise multiple comparison procedures were performed with Dunn’s test. Differences between induced damage and remnant damage were obtained per (a) and (b), and . Student’s t-test versus control was applied for data in (c) and (d), and . Images in Supplementary Figure 3.
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