Human Placenta-Derived Mesenchymal Stem Cells Reduce Mortality and Hematoma Size in a Rat Intracerebral Hemorrhage Model in an Acute Phase

Figure 1

Conceptual illustrations of the experimental protocol. (a) Schematic diagrams of a rat intracranial hemorrhage (ICH) model with the administration of human placenta-derived mesenchymal stem cells (PD-MSCs). ICH was induced by the infusion of bacterial collagenase type IV (0.1 U, 1 μL) into the striatum. PD-MSCs (1 × 10⁶ cells) were injected slowly via the tail vein at 1 hour after ICH. (b–d) Brief timeline of the experimental procedures which represent all the animal cohorts used and analyses performed: (b) mortality rate and hematoma volume; (c) ventricle size, hemispheric enlargement, and neuronal death; and (d) Western blot and real-time PCR. (e) Flow diagram which described how rats were used in a stepwise fashion.

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