Human Placenta-Derived Mesenchymal Stem Cells Reduce Mortality and Hematoma Size in a Rat Intracerebral Hemorrhage Model in an Acute Phase

Figure 2

Effect of human placenta-derived mesenchymal stem cell (PD-MSC) administration on mortality, hematoma volume, ventricular enlargement, and brain edema of the rats at 24 hours after the induction of intracranial hemorrhage (ICH). (a) Mortalities in each group at 24 hours after ICH ( from each group). (b) Digital photographs showing location of a core hemorrhagic region at 0.2 mm from the bregma. Scale bar = 1 mm. (c) The bar graphs represent the hematoma volume of the vehicle-treated and the PD-MSC-treated groups at 24 hours after ICH induction. The volume of hematoma is expressed as the proportion in the total brain area (%). Data are mean + SEM; n = 7-8 from each group (ICH-Veh, ; ICH-MSC, ), . (d) Representative images of cresyl violet staining depicting a coronal whole-brain section at rostral-caudal levels from +2.04 to −5.52 from the bregma. Scale bar = 1 mm. (e) The bar graphs represent the ventricular size of the vehicle-treated and the PD-MSC-treated groups at 24 hours after ICH induction. The size of the lateral ventricle is expressed as the proportion in the total brain area (%). Data are mean + SEM; n = 7-8 from each group (ICH-Veh, ; ICH-MSC, ), . (f) The bar graphs represent the degree of the hemispheric enlargement of the vehicle-treated and the PD-MSC-treated groups at 24 hours after ICH induction. The hemispheric enlargement is expressed as the percentage of increase in hemispheric size compared with that of the contralateral hemisphere. Data are mean + SEM; n = 7-8 from each group (ICH-Veh, ; ICH-MSC, ), .

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