Chronic Inflammation May Enhance Leiomyoma Development by the Involvement of Progenitor Cells

Figure 7

Expression of selected cytokines in myometrium progenitor cells (MPCs) and leiomyoma progenitor cells (LPCs). (a) Quantification of mRNA expression in MPCs and LPCs was calculated with the 2^−ΔCt method, where ΔCt = Ct (gene of interest) − Ct (housekeeping gene). ΔCt was calculated for the selected genes on 12 different cultures. Subsequently, mean ± SD from three independent experiments in triplicates was calculated for LPCs and displayed. LPCs versus MPCs. (b) ELISA test; the levels measured in MPCs were considered as 100% and those detected in LPCs accordingly calculated; LPCs versus MPCs. (c) Selected Th1/Th17 pathway molecules evaluated by RT-PCR. Quantification of mRNA expression in MPCs and LPCs was calculated with the 2^−ΔCt method, where ΔCt = Ct (gene of interest) − Ct (housekeeping gene). ΔCt was calculated for the selected genes on 12 different cultures. Subsequently, mean ± SD from three independent experiments in triplicates was calculated for LPCs and displayed.

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