Comparison of the MicroRNA Expression Profiles of Male and Female Avian Primordial Germ Cell Lines

Figure 13

Inhibition of the gga-miR-302b-5p and gga-miR-302b-3p expression in PC PGC lines using anti-miR-302b vectors. Chicken FS101 and FS111 PC PGC lines were transfected with anti-miR-302b-5P and anti-miR-302b-3P vectors using a siPORT transfection agent. The proliferation rate of the treated and control cells was measured using CCK-8 reagent every day, for 3 days. (a) The inhibition of gga-miR-302b-5P significantly reduced the proliferation rate of the FS101 PGCs, which was revealed in a significant increase in doubling time of FS101 PC PGCs (). (b) The inhibition of gga-miR-302b-5P significantly reduced the proliferation rate of the FS111 PGCs, which was revealed in a significant increase in doubling time of FS111 PC PGCs (). PC PGCs: Partridge colour Hungarian chicken embryo-derived primordial germ cells.

(a)

(b)