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Figure 3: Immunofluorescence staining of 4ZP and 5ZP GFP PGCs. (a) Confocal images of GFP-expressing (green) 4ZP PGCs. (b) Higher magnification of 4ZP PGCs showing CVH expression (CVH (red), GFP (green), and NS (blue)) (white square indicates the cells on (a)). (c) Confocal images of GFP-expressing (green) 5ZP PGCs. (d) Higher magnification of 5ZP PGCs showing CVH expression (CVH (red), GFP (green), and NS (blue)) (white square indicates the cells on (c)). For nuclear staining (NS), we used TO-PRO-3 (blue). Scale bars: 25 μm (a, c) and 5 μm (b, d). GFP PG cells: green fluorescent protein- (GFP-) expressing transgenic chicken embryo-derived primordial germ cells.