Comparison of the MicroRNA Expression Profiles of Male and Female Avian Primordial Germ Cell Lines

Table 1

The efficiency of the establishment of chicken primordial germ cell (PGC) lines. (a) 21 PGC lines from Partridge colour Hungarian chicken breed (PC lines) were established. We got 4 (19%) female PGC lines. (b) 10 GFP-expressing White Leghorn embryo-derived chicken PGC lines (GFP lines) were established. 2 (20%) female PGC lines were detected. PC PGC lines: Partridge colour Hungarian chicken embryo-derived primordial germ cell lines, GFP PGC lines: green fluorescent protein- (GFP-) expressing transgenic chicken embryo-derived primordial germ cell lines.

(a)

PC chicken PGC line isolation	Number	%
Used embryos	74	100
Established PGC lines	21	28
Frozen PGC lines	21	28
Female cell lines	4	19

(b)

GFP chicken PGC line isolation	Number	%
Used embryos	20	100
Established PGC lines	10	50
Frozen PGC lines	10	50
Female cell lines	2	20