Location, Isolation, and Identification of Mesenchymal Stem Cells from Adult Human Sweat Glands

Figure 3

In vitro tissue culture from detached ahSG solenoid bulbs. (a) Typical morphology of different cells growing out from an ahSG fragment. The boxed area was magnified to visualize the fibroblast-like cells and epithelioid cells wrapped around them. (b) Double immunofluorescence of the primary cells growing out from the ahSG fragment using antibodies against CK15 and α-SMA. The cells were stained with antibodies to K15 (red) and α-SMA (green) with cell nuclei stained by Hoechst 33342 (blue). (c) FCM showing α-SMA expression by the hSG secretory cells. (a) 40x and the box is 100x. (b) 400x.

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