Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation

Figure 1

Effect of culture media on the morphology, cell yield, and granule formation of the cells generated from CD133⁺ hematopoietic stem cells (HSCs) in a matrix after seven weeks in culture. (a) Micrographs showing the morphology of generated cells in the test media. White arrows highlight typical MCs. Black arrow highlights the larger-sized MCs found in StemPro (Ser1–7). (b) Metachromatic staining of cytoplasmic granules in the generated cells. A few typical MCs are highlighted by white arrows. In StemPro, some cells were hypogranulated, as shown by the black arrow. (c) Cell yields in the culture media. Cell yield is defined as the ratio of the cells collected at the end of the culture period to the seeded cells. (d) Histamine granule formation in the generated cells. Data are represented as mean ± SD; . indicates .

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