Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation

Figure 3

Effect of serum on cell yield, granule formation, and marker expression of the cells generated from CD133⁺ hematopoietic stem cells (HSCs) in a matrix after seven weeks in culture. (a) Metachromatic staining of cytoplasmic granules in the cells generated with serum added in the first two and last weeks of culture. Some of the generated cells were bi- or multinucleated, as highlighted by a white arrow. (b) Cell yield of generated cells with serum added at different time points. The ratio of cells collected at the end of the culture period to the cells seeded at the beginning of culture was reported as relative cell yield. (c) Histamine granule formation in the generated cells. (d) Expression of c-kit by generated cells. In all cases, StemSpan medium was used. Data are represented as mean ± SD; . indicates between the media tested.

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