Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation

Figure 4

Effect of serum-free media on fibroblast growth and characteristics. (a) Morphology of fibroblasts at six days postseeding in serum-free media. Arrows show some of the typical fibroblasts. The rest of the experiments were performed ten days postseeding. (b) Fibroblast yield in the culture media. The ratio of the number of collected cells to the number of seeded cells is shown as relative cell yield. (c, (i) and (ii)) Proliferation of fibroblasts in the culture media. Solid histogram shows the cells analyzed on the day of seeding (nondividing cells). (d, (i) and (ii)) Expression of CD90 by fibroblasts in the culture media. Expression of CD90 (open histogram) was compared to an isotype control (solid histogram). (e) Release of SCF and IL-6 by fibroblasts. In all cases, serum-supplemented M199 was taken as the standard cell culture medium. Data are represented as mean ± SD; . indicates between M199 and the test media. † indicates between the test culture media.