Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation

Figure 5

Effect of culture media on endothelial cell (EC) growth and characteristics. (a) Micrographs depicting poor survival of ECs at three days postseeding in serum-free media. Typical detached or dead ECs are highlighted by arrows. (b) Micrographs depicting survival of ECs at six days postseeding in serum-supplemented media. Typical cells are highlighted by arrows. The rest of the experiments were performed 12 days postseeding. (c) EC yields in the serum-supplemented media. Ratio of the number of collected cells to the number of seeded cells is shown as relative cell yield. (d, (i) and (ii)) Proliferation of ECs in the serum-supplemented media. Solid histogram shows the nondividing cells. (e, (i) and (ii)) Expression of CD31 by ECs in the serum-supplemented media. The expression of CD31 (open histogram) was compared to an isotype control (solid histogram). (f) Release of IL-6 by ECs. In all cases, serum-supplemented M199 was taken as the standard cell culture medium. Data are represented as mean ± SD; . indicates between M199 and test media. † indicates between test culture media.