Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation

Figure 6

Expression of tryptase and chymase by generated cells from CD133⁺ hematopoietic stem cells (HSCs) in a collagen matrix after seven weeks in culture. StemSpan with serum in the seventh week of culture was used as culture media. Representative density plots and histograms of the marker expression are shown. Expression of tryptase and chymase by c-kit-positive cells (gray histogram) is compared with the isotype control (black histogram).