An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro

Figure 2

bFGF inhibits the differentiation of cESCs in vitro. (a) The inhibition of FGFR1 promoted the differentiation. The primary cESCs in the complete medium were treated with an effective FGFR1 inhibitor PD173074. Differentiated cells were highlighted in the yellow box. (b) The inhibition of FGFR1 led to an increase in the mRNA level of three germ layer marker genes. implies: compared to control group, there is a extremely remarkable difference (). Quantitative RT-PCR of GATA4, ALB, CDX2, PAX6, SOX1, and β-actin was performed. (c) The inhibition of FGFR1 suppressed the formation of the clone. Crystal violet staining of primary cESCs in the presence with PD173074. (d) The colony area stained by crystal violet was calculated by Image-Pro Plus (). (e) The Image-Pro Plus analysis of the clone number (). All results are expressed as the mean ± SD of three independent experiments. Scale bar: 100 μm.

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