Single-Cell Gene Expression Analysis and Evaluation of the Therapeutic Function of Murine Adipose-Derived Stromal Cells (ASCs) from the Subcutaneous and Visceral Compartment

Figure 1

Single-cell transcriptional analysis of S-ASCs and V-ASCs. (a) At the single-cell level, S-ASCs and V-ASCs showed significant differences in genes related to neoangiogenesis and tissue remodeling (such as Ccl2, Hif1α, Fgf7, and Igf). Gene expression is shown on a color scale from yellow (high expression) to blue (low expression). (b) Whisker plots show qPCR cycle threshold for each gene. Individual dots each represent a cell/gene qPCR reaction, (c) clustering of S-ASCs and V-ASCs based on their gene expression patterns. (d) Pie charts represent the fractions of the ASCs that make up the respective clusters.

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