Foxc2 and BMP2 Induce Osteogenic/Odontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla

Figure 2

Lentiviral transfection efficiency. (a) Forty-eight hours after lentiviral transfection, all four group cells presented more than 90% GFP expression percentage, which means the lentivirus plasmid-mediated gene transfection efficiencies of the four groups cells are high. (b) Four days after transfection, the Foxc2 expression in the mRNA level was significantly upregulated () in SCAP-Foxc2 and SCAP-Foxc2-BMP2. Similarly, the BMP2 expression in the mRNA level was significantly upregulated () in SCAP-BMP2 and SCAP-Foxc2-BMP2. (c, d) Four days after transfection, western blot results further confirmed that the Foxc2 expression in the peptide level was correspondingly increased () in SCAP-Foxc2 and SCAP-Foxc2-BMP2. The BMP2 expression in the peptide level was also significantly () increased in SCAP-BMP2 and SCAP-Foxc2-BMP2. .

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