Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2018 (2018), Article ID 2405698, 12 pages
https://doi.org/10.1155/2018/2405698
Research Article

B7-H1 Expression Is Required for Human Endometrial Regenerative Cells in the Prevention of Transplant Vasculopathy in Mice

1Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China
2Tianjin General Surgery Institute, Tianjin, China
3Department of Vascular Surgery, Tianjin Fourth Central Hospital, Tianjin, China
4Faculty of Medicine, University of Toronto, Toronto, ON, Canada
5Department of Endocrinology, Tianjin Medical University General Hospital, Tianjin, China

Correspondence should be addressed to Hao Wang; moc.liamtoh@272acgnawh

Received 11 September 2017; Revised 2 January 2018; Accepted 17 January 2018; Published 14 March 2018

Academic Editor: Jun Liu

Copyright © 2018 Kui Ye et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Vasculopathy is one of the primary pathological changes in chronic rejection of vascularized allograft transplantation. Endometrial regenerative cells (ERCs) are mesenchymal-like stromal cells with immunosuppressive effect. B7-H1 is a negative costimulator that mediates active immune suppression. The aim of this study was to investigate the requirement of B7-H1 in the immunoregulation of ERCs in preventing transplant vasculopathy of aorta allografts. The results showed that B7-H1 expression on ERCs was upregulated by IFN-γ in a dose-dependent manner and it was required for ERCs to inhibit the proliferation of peripheral blood mononuclear cells (PBMCs) in vitro. ERCs could alleviate transplant vasculopathy, as the intimal growth of transplanted aorta was limited, and the preventive effects were correlated with an increase in the percentages of CD11c+MHC class IIlowCD86low dendritic cells, CD68+CD206+ macrophages, and CD4+CD25+Foxp3+ T cells, as well as a decrease in the percentages of CD68+ macrophages, CD3+CD4+ T cells, CD3+CD8+ T cells, and donor-reactive IgM and IgG antibodies. Moreover, overexpression of B7-H1 by IFN-γ can promote the immunosuppressive effect of ERCs. These results suggest that overexpression of B7-H1 stimulated by IFN-γ is required for ERCs to prevent the transplant vasculopathy, and this study provides a theoretical basis for the future clinical use of human ERCs.