B7-H1 Expression Is Required for Human Endometrial Regenerative Cells in the Prevention of Transplant Vasculopathy in Mice

Figure 7

B7-H1 is required for ERCs to decrease the percentage of donor-reactive antibodies in sera. The sera were collected from B6 recipients at posttransplant day 8, 1 : 20 dilution in PBS, then incubated with splenocytes of BALB/c mouse for 30 min at 37°C, and followed by double staining with either anti-mouse CD4 and IgM antibodies or anti-mouse CD4 and IgG antibodies for the FACS analysis. (a) Dot plots of CD4⁺IgM⁺ antibodies and CD4⁺IgG⁺ antibodies in each group. (b) Percentage of CD4⁺IgM⁺ antibodies and CD4⁺IgG⁺ in each group. Values were presented as mean ± SEM; statistical analysis was done by one-way ANOVA followed by the LSD test, .

(a)

(b)