Diabetic Ephrin-B2-Stimulated Peripheral Blood Mononuclear Cells Enhance Poststroke Recovery in Mice

Figure 10

Ephrin-B2 stimulates the expression of genes involved in angiogenesis and inflammation of human diabetic PB-MNC. (a) Genes showing upregulated or downregulated expression after one hour (left graph) or four hours (right graph), in three or more of the total five samples per time point, are listed in descending order according to the average change in expression levels following ephrin-B2 stimulation. For a human gene to be considered significantly upregulated or downregulated by ephrin-B2, expression in the human array had to be at least twofold higher or lower, respectively. Figures to the right of the outer edge of bars indicate the number of patients in whom gene expression was significantly changed at both time points (). (b) Percentage adhesion of ephrin-B2-stimulated PB-MNC to HUVEC monolayer was increased 3.5-fold in adherent cells (/group). Data are presented as % adhesion ± HdSD. (c) Capillary-like tube formation assay on Matrigel. The number of branch points was significantly greater in the PB-MNC+ compared to PB-MNC groups (/group). Data are presented as mean ± SD. ; .

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