Review Article
Three-Dimensional Bioreactor Technologies for the Cocultivation of Human Mesenchymal Stem/Stromal Cells and Beta Cells
Table 1
Process-relevant parameters for human mesenchymal stem/stromal cells (hMSCs) and islets or beta cells based on our own data and the literature.
| | hMSC | hMSC-TERT | Islet | 1.1B4 | MIN6 |
| | Biological parameter | μ [d−1] | 0.33–1.1 | 0.4–0.7# | 0.11 [49] | 0.84 | 0.19–0.23 [100] | | tD [h] | 15–50 | 24–42 | 151 | 20 | 72–88 | | qGLC [pmol cell−1 h−1] | 0.13–0.7 | 0.02–0.5# | n.d. | 0.08# | n.d. | | qox [fmol cell−1 h−1] | 90–100 [77] | 300 [74] | 23–139 [89] | n.d. |
| | Process parameter | Seeding density [cells cm−2] | 1000–10,000# | Depends on reaggregation method | 5000–10,000# | >80,000 | | Final cell density [cells cm−2] | 5·104 (dynamic)#
2·105 (static)# | 1·105 (static)# | 2-3·105 (static)# | | Max. working volume [L] | 35 | 2.4# | <0.1 | | pH [−] | 7.0–7.4 | | T [°C] | 30–37 | | kLa [h−1]+ | 0.6 | 4.4 | 0.2 | n.d. | | Shear sensitivity τ [N cm−2] | 1.2·10−5 | Mostly static cultivation with low shear | | Growth surface material | TC-treated polystyrene, micro- and macrocarrier with different coatings | Mainly TC-treated culture vessels (polystyrene) | | Aggregate formation | Possible, but lower tendency | Strong tendency | | Culture media | SCM, SFM, CDM | SCM with 10–20% serum | | Glucose concentration [mM] | Usually 5.5, 5–25 | 5.8 recommend, 5–25 |
|
|
Abbreviations: μ: growth rate; tD: doubling time; qGLC: glucose consumption; qox: oxygen consumption; kLa: oxygen transfer coefficient; TC: tissue culture; SCM: serum-containing medium; SFM: serum-free medium; CDM: chemically defined medium; n.d.: not determined. Murine origin; #own data; +aeration with air. |
