Icariin Promotes the Migration of BMSCs In Vitro and In Vivo via the MAPK Signaling Pathway

Figure 5

Effect of MAPK inhibitors on the wound-healing assay and rhodamine-phalloidin staining to determine the role of the MAPK signaling pathway in ICA-induced migration. (a) Activation of the MAPK signaling pathway was required for ICA-induced BMSC migration in the scratch wound-healing assay. BMSCs were treated with ICA (1 μM) in the presence of three inhibitors. The wounds were evaluated at 12 and 24 h after scratching. (b) Statistical data analysis of the remaining wound area. (c) ICA induces actin stress fiber formation by upregulating the MAPK signaling pathway. BMSCs were pretreated with the three inhibitors for 1 h. Then, rhodamine-phalloidin staining was performed after treatment with ICA.

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