Potential of iPSC-Derived Mesenchymal Stromal Cells for Treating Periodontal Disease

Figure 2

Trilineage differentiation potential of the miPSC-MSC. (a) Representative image of the osteogenic differentiation potential of miPSC-MSC-like cells visualised by Alizarin Red staining of mineralised deposits formed after 4 weeks of culture in osteogenic inductive media. Real-time PCR analysis of the relative expression of genes associated with osteogenesis, bone sialoprotein-2 (BSP2), and osteopontin (OPN). (b) Representative image of the adipogenic potential of miPSC-MSC visualised by Oil Red-O staining for lipid formation after 4 weeks of culture in adipogenic induction medium. Real-time PCR analysis of the relative expression of genes associated with adipogenesis CEBP-α and PPAR-γ2. (c) Representative image of the chondrogenic differentiation potential of miPSC-MSC visualised by anticollagen type II antibody staining on the chondrocyte pellets formed after culture in chondrogenic media for 4 weeks. Real-time PCR analysis of the relative expression of genes associated with chondrogenesis collagen type II (COL-2) and SOX9. The data represent the mean expression values normalized to the housekeeping gene β-actin ± SEM ( unpaired t-test).

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