N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells
(a-e) Neurogenic differentiation of MenSCs promotes N-cad expression. Immunofluorescence staining (a, b) and RT-PCR (c) were used to determine the expression of N-cad in MenSCs with or without neurogenic induction. The percentage of N-cad-positive cells (d) and the relative mRNA expression levels of N-cad (e) in MenSCs after neurogenic differentiation. (f) Inhibition of N-cad expression affects the migration of NPCs in vivo. The GFP-positive embryos and brains were collected and imaged under a stereo fluorescence microscope at E20, and the subsequently prepared cryosections clearly showed that the decrease in GFP intensity was caused by abnormal retention of GFP-positive NPCs at the ventricular zone. .