The Myc/Max/Mxd Network Is a Target of Mutated Flt3 Signaling in Hematopoietic Stem Cells in Flt3-ITD-Induced Myeloproliferative Disease

Figure 2

Myeloid and multipotent progenitors have altered Myc network genes in Flt3-ITD mice. The expression of Myc network genes was carried out with reverse transcription quantitative PCR (RT-Q-PCR) in sorted subpopulations of murine hematopoietic stem and progenitor cells. Fold induction was calculated as a ratio of Flt3-ITD : WT samples. Statistical analysis was performed with Student’s -test on log-converted values (). Significance was shown in many of the described altered expression levels; however, some changes showed low values but did not quite reach statistically significance due to variances in the expression levels.