MicroRNA-132, Delivered by Mesenchymal Stem Cell-Derived Exosomes, Promote Angiogenesis in Myocardial Infarction

Figure 2

Internalization of miR-132-electroporated exosomes and detection of target gene RASA1. (a) Confocal images of DiI-labelled exosomes taken up by HUVECs. Scale bar: 20 μm. (b, c) HUVECs were incubated with miR-132 mimics or inhibitor-electroporated exosomes for 2 h. The relative expression level of miR-132 and its target gene RASA1 was detected by RT-PCR (). (d) 293T was cotransfected with miR-132 mimics or NC and firefly luciferase reporter plasmid containing wild-type or mutant-type 3UTR of RASA1. After incubation for 48 h, the firefly luciferase activity of each sample was detected and normalized to the Renilla luciferase activity (). The data represent the mean ± SEM of triplicates. , , .

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