Research Article
The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation
Table 1
The primers used for RT-qPCR analysis. ALP: alkaline phosphatase; Runx2: runt-related transcription factor 2; and Ocn: osteocalcin.
| | Target gene | Primers | Sequence | Fragment size (bp) |
| | ALP | Forward primer | 5- ATAACGAGATGCCACCAGAGG-3 | 140 bp | | Reverse primer | 5- TTCCACATCAGTTCTGTTCTTCG-3 |
| | RUNX2 | Forward primer | 5- CTACCCAGCCACCTTTACCTAC-3 | 190 bp | | Reverse primer | 5- GAACTGATAGGATGCTGACGAAG-3 |
| | OCN | Forward primer | 5- AGGAGGGCAATAAGGTAGTGAAC-3 | 147 bp | | Reverse primer | 5- AGGCGGTCTTCAAGCCATAC-3 |
| | β-actin | Forward primer | 5- GAGACCTTCAACACCCCAGC-3’ | 263 bp | | Reverse primer | 5- ATGTCACGCACGATTTCCC-3 |
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