IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway

Figure 1

IL-1β-induced MSC migration and invasion. (a) Cell wound healing assay for IL-1β-stimulated mesenchymal stem cells in the presence or absence of 2 μg/ml IL-1RA (IL-1β inhibitor) at 6 and 18 hours. Scale bars = 500 μm. (b) Graph indicates the migration ability of stem cells into the wound area. Data are shown as mean ± SD (, versus control, versus IL-1β). (c) Cell invasion assay; cultures were treated with IL-1β in the presence or absence of IL-1RA for 18 hours. (d) Quantitative data in invasion assay are presented as mean ± SD of triplicate samples. Data are shown as mean ± SD (, , ). (e) Cell viability assay for IL-1β stimulation at different concentrations. Data were quantified by multimode microplate readers. Data are shown as mean ± SD (, ). (f) MTT cell proliferation data for IL-1β stimulation. Data were quantified by multimode microplate readers. Data are shown as mean ± SD ().

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