IL-1β-Induced Matrix Metalloprotease-1 Promotes Mesenchymal Stem Cell Migration via PAR1 and G-Protein-Coupled Signaling Pathway

Figure 3

MMP-1 is required for IL-1β-induced MSC migration. (a) MMP-1 protein expression measured by ELISA. Pretreatment with both TIMP1 and TIMP2 together at a concentration of 45 nM reduced MMP-1 concentration in the culture. Data are shown as mean ± SD (, versus control, versus IL-1β). (b) Cell wound healing assay. Cultures were treated with MMP inhibitors TIMP1 and TIMP2 and MMP-1 inhibitor GM6001 at concentrations of 45 nM, 45 nM, and 50 nM, respectively, as indicated. Scale bars = 500 μm. (c) Quantitative graph showing the migration ability of stem cells into the wound area at 6 and 18 hours. Data are shown as mean ± SD (, versus control, versus IL-1β at 18 hours). (d) Cell wound healing assay for IL-1β-stimulated MSCs after MMP-1 siRNA transfection (scale bars = 500 μm). (e) Quantitative graph showing the migration ability of MMP-1 siRNA-transfected stem cells into the wound area at 6 and 18 hours. Data are shown as mean ± SD (, versus control, versus IL-1β at 18 hours). (f) Western blot results of the phosphorylated ERK 1/2 from the lysates of cells pretreated with TIMP1/2 and GM6001.

(a)

(b)

(c)

(d)

(e)

(f)