Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells

<div>Osteogenic induction evaluated with Alizarin red S staining after 21 days in osteogenic media (a) or basal (b) media. Calcium nodules were seen in osteogenic medium (a), but not in basal medium (B). Scale bars: 200 <i>μ</i>m; inset, 100 <i>μ</i>m. (c) Graph showing the osteogenic gene (<i>RUNX2</i>, <i>SPP1</i>, and <i>BGLAP</i>) expression compared between osteogenic medium and its respective basal cultures. The level of expression of each target gene was normalized to <i>GAPDH</i>. <svg height="10.7395pt" id="M19" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 56.4993 10.7395" width="56.4993pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" id="g50-43"></path></g><g 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146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z" id="g113-47"></path></g><g transform="matrix(.013,0,0,-0.013,38.204,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z" id="g113-49"></path></g><g transform="matrix(.013,0,0,-0.013,44.444,0)"><path d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z" id="g113-54"></path></g></svg>.</span> <i>RUNX2</i>: runt-related transcription factor 2; <i>SPP1</i>: osteopontin; <i>BGLAP</i>: osteocalcin.</div>

Stem Cells International

fig4

Figure 4

Figure 4: Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells 