Comparative Analysis of Human Adipose-Derived Mesenchymal Stem Cells from Orbital and Abdominal Fat

Figure 3

Osteogenic differentiation of orbital and abdominal ASCs towards osteogenesis. (a) Microscopic photograph of ASCs cultured in osteogenic medium for 21 days and stained with alizarin red stain. Control cells were grown in DMEM/F12 medium for the same period of time. (b) Quantitation analysis of mineralization by determining the amount of dye extracted and measured at absorbance 405 nm wavelength. (c) Real-time PCR analysis for genes involved in osteogenesis, such as BMP2 and SP7. Results are presented as means ± standard error mean (SEM). and .

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