Timing of Wnt Inhibition Modulates Directed Differentiation of Medial Ganglionic Eminence Progenitors from Human Pluripotent Stem Cells

Figure 2

Gene expression analysis of NPC (D21) generated by the AdD protocol. NSC (D7) were treated for 14 days with either SHH alone, 200 ng/ml SHH (SHH, 200), 500 ng/ml SHH (SHH, 500), or in combination with 100 ng/ml FGF8 (SHH, 200 + FGF8) or 2 mM IWP-2 (SHH, 200 + IWP-2; SHH, 500 + IWP-2), and with 1.5 mM purmorphamine (PUR).