Adipose-Derived Stromal Vascular Fraction/Xenohybrid Bone Scaffold: An Alternative Source for Bone Regeneration

Figure 7

Osteoblast differentiation of ASCs and SVF on SmartBone. At 60 days, in ctrl condition, collagen I (COLL I) was weakly positive on SmartBone cultured with ASCs (a), whereas in culture with SVF, COLL I was expressed (c). Both in cultures with ASCs and SVF with osteogenic medium (OM), COLL I markedly stained the new tissue on SmartBone (b, d). Osteocalcin (OCN) was weakly positive in ctrl (e, g), and it was highly expressed with OM, mainly on the periphery of the newly formed tissue (f, h). TGFb stained osteoblasts at the margin of the newly formed tissue both with and without OM, with ASCs and SVF (i–l). Magnification: 20x.