Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells

Table 1

Primers used for real-time PCR.

Gene	Forward primer (5 to 3)	Reverse primer (5 to 3)
GAPDH	5-AGAAAAACCTGCCAAATATGATGAC-3	5-TGGGTGTCGCTGTTGAAGTC-3
β-Actin	5-GACTTAGTTGCGTTACACCCTTTC-3	5-GCTGTCACCTTCACCGTTCC-3
Col2A1	5-GGCAATAGCAGGTTCACGTACA-3	5-CGATAACAGTCTTGCCCCACTT-3
SOX 9	5-AGCGAACGCACATCAAGAC-3	5-GCTGTAGTGTGGGAGGTTGAA-3
TβRI	5-ATTACCAACTGCCTTATTATGA-3	5-CATTACTCTCAAGGCTTCAC-3
TβRII	5-ATGGAGGCCCAGAAAGATG-3	5-GACTGCACCGTTGTTGTCAG-3
TβRIII	5GTGTTCCCTCCAAAGTGCAAC-3	5-AGCTCGATGATGTGTACTTCCT-3

GAPDH: glyceraldehyde-3-phosphate dehydrogenase; COL2A1: collagen type II; SOX9: SRY- (sex determining region Y-) box 9; TβRI/II/III: recombinant human transforming growth factor-β receptor type I/II/III.