Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells
Transfection with SOX2 plasmid increased SOX2 mRNA expression and did not affect PBMC viability. PBMCs were transfected one day after plating with increasing concentrations of either the plasmid pEP4 E02S EN2K carrying the pluripotency genes SOX2, KLF4, NANOG, and OCT3/4 (En2k) or pCAGGS plasmid carrying only SOX2 (Sox2). The panels show SOX2 mRNA expression as determined by qPCR (a) and the viability as determined by Trypan blue in the transfected cells by either plasmids (b) two days post transfection. Data are presented as mean ± SEM of . The symbol denotes a significantly higher Sox2 group value than En2k value (-test). The symbol # indicates a significant difference to the respective cells which received 0.2 μg DNA (Student’s -test after ANOVA ).
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