Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells
SOX2 overexpression induces proliferation in RMCMO. Immunofluorescence of Ki67 expression (red) in normal RMCMO (a) or RMCMO generated after transfection of PBMCs with SOX2 (b). Cells were also immunostained with anti-CD14 (green) as a monocyte-specific marker. Nuclei were stained with DAPI (blue). (c) Quantification of Ki67 in SOX2-overexpressing RMCMO. (d) RMCMO cell count (relative to control value set arbitrarily at 1) 4 days after SOX2-transfection. Control = empty vector-transfected RMCMO. Data () are expressed as mean ± SEM. Statistical analysis was performed by unpaired Student’s -test.
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