Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells
SOX2-overexpression improves hepatic cell function in RMCMO-derived neohepatocytes. RMCMO derived from SOX2-transfected PBMCs were cultured in hepatocyte conditioning medium for 2 weeks before subjecting them to analysis of cytochrome P450 (CYP) isoforms 1A1/2, 2D6, and 3A4, urea metabolism, and glucose metabolism. Activities and secretion levels were normalized to protein content (mg) of each sample. Data are presented as mean ± SEM of . Statistical analysis between SOX2-transfected and nontransfected control cells was performed by unpaired Student’s -test. Pyr, pyruvate; Lac, lactate.
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