Stem Cells International / 2018 / Article / Fig 4

Research Article

Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells

Figure 4

SOX2-overexpression improves hepatic cell function in RMCMO-derived neohepatocytes. RMCMO derived from SOX2-transfected PBMCs were cultured in hepatocyte conditioning medium for 2 weeks before subjecting them to analysis of cytochrome P450 (CYP) isoforms 1A1/2, 2D6, and 3A4, urea metabolism, and glucose metabolism. Activities and secretion levels were normalized to protein content (mg) of each sample. Data are presented as mean ± SEM of . Statistical analysis between SOX2-transfected and nontransfected control cells was performed by unpaired Student’s -test. Pyr, pyruvate; Lac, lactate.

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.