Effect of a Combination of Prednisone or Mycophenolate Mofetil and Mesenchymal Stem Cells on Lupus Symptoms in MRL.Fas^lpr Mice

Figure 7

Effect of immunosuppressants on MSC functions in vitro. MSCs were pretreated with 100 μM of PD, PDL, MMF, or MPA for 24 h. MSCs (2 × 10⁴ cells/well) were added to the upper chamber and allowed to migrate to the lower chamber containing CXCL-10 (100 ng/ml). MSCs that migrated to the lower side of inserts were stained with crystal violet (a). Immunosuppressant-pretreated MSCs (0.1 × 10⁴ cells/well) were mixed with T cells (1 × 10⁵ cells/well) in a 96-well plate. Concanavalin A (ConA) was added at 1 μg/ml, and the proliferation of (b) and IFN-γ production (c) by T cells were measured 72 h later. versus control.

(a)

(b)

(c)