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Stem Cells International
Volume 2018, Article ID 4527929, 12 pages
https://doi.org/10.1155/2018/4527929
Research Article

Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human Hematopoietic/Progenitor Cells

1Department of Biomedical Engineering, Inje University, Gimhae, Gyeongsangnam-do, Republic of Korea
2Department of Biochemistry and Cancer Research Institute, Kosin University College of Medicine, Busan, Republic of Korea
3Laboratory for Cancer & Stem Cell Biology, Plant Engineering Institute, Sejong University, Seoul, Republic of Korea
4Department of Internal Medicine, Kosin University College of Medicine, Busan, Republic of Korea
5Department of Health Science and Technology/Cardiovascular and Metabolic Disease Center/Institute of Aged Life Redesign/UHARC, Inje University, Gimhae, Republic of Korea

Correspondence should be addressed to Jung-Woog Shin; moc.liamg@nihshcemoib

Received 24 October 2017; Accepted 31 December 2017; Published 27 February 2018

Academic Editor: Kevin D. Bunting

Copyright © 2018 Yun Gyeong Kang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ex vivo expansion of hematopoietic stem/progenitor cell (HSPC) has been investigated to improve the clinical outcome of HSPC transplantation. However, ex vivo expansion of HSPCs still faces a major obstacle in that HPSCs tend to differentiate when proliferating. Here, we cocultured HSPCs with mesenchymal stem cells (MSCs) and divided the HSPCs into two fractions according to whether they came into adherent to MSCs or not. Additionally, we used hydrostatic pressure (HP) to mimic the physical conditions in vivo. Even nonadherent cells expanded to yield a significantly larger number of total nucleated cells (TNCs), adherent cells maintained the HSPC phenotype (CD34+, CD34+CD38, and CD133+CD38) to a greater extent than nonadherent cells and had superior clonogenic potential. Moreover, applying HP significantly increased the number of TNCs, the frequency of the immature HSPC phenotype, and the clonogenic potential. Furthermore, the genetic markers for the HSPC niche were significantly increased under HP. Our data suggest that the nonadherent fraction is the predominant site of HSPC expansion, whereas the adherent fraction seems to mimic the HSPC niche for immature cells. Moreover, HP has a synergistic effect on expansion and functional maintenance. This first study utilizing HP has a potential of designing clinically applicable expansion systems.