Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human Hematopoietic/Progenitor Cells
Clonogenic potential of expanded cells. (a) CFU-GEMM, (b) BFU-E, (c) CFU-GM, and (d) total CFU counts after 14 days in culture, using cells cultured for 4 days under various conditions (, ). (e) Morphological evaluation of CFUs in the H_M_A_HP group after 4 days in culture. Images are representative of three data sets (CFU-GEMM, bar = 200 μm; BFU-E and CFU-GM, bar = 100 μm). CFU-GEMM: colony-forming units-granulocyte, erythrocyte, monocyte, and megakaryocyte; BFU-E: burst-forming unit-erythroid; CFU-GM: colony-forming unit-granulocyte and macrophage.
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