Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human Hematopoietic/Progenitor Cells
Gene expression of HSPC niche markers. The relative gene expression of HSPC niche markers (ANGPT1, ANGPT2, JAG1, OPN, RUNX2, SDF1, TPO, and VCAM1) was confirmed by harvesting MSCs cocultured with HSPCs with or without the application of HP. Additionally, single cultured MSCs were used (MSCs without HSPC) as a control (, ). ANGPT1: angiopoietin 1; ANGPT2: angiopoietin 2; JAG1: jagged 1; OPN: osteopontin; RUNX2: runt-related transcription factor 2; SDF1: stromal cell-derived factor 1; TPO: thrombopoietin; VCAM1: vascular cell adhesion molecule 1.